Friday, May 8, 2015

Explain Biochemical test for Glucose, Protein and Lipids and Their positive test results.

To test for simple carbohydrates such as glucose, you
would use Benedict's solution.  Benedict's solution is an indicator for simple sugars.
If the color is an aqua blue, then the result is negative for simple sugars.  If you get
a yellow/green/red color, then the result is positive for simple sugars.  Unlike many
indicators, Benedict's solution does not work at room temperature and must be heated
prior to taking results.


To test for a complex carbohydrate
such as starch, you would use Iodine solution.  In the presence of starch, the iodine
would be black/blue.


To test for proteins (amino acids, the
building blocks of proteins), you would use biuret solution.  A negative test would show
a blue color and a positive test would result in a violet blue to a pink purple
color.


To test for lipids you would use Sudan IV.  A
negative result would be dark red and a positive test for lipids would show a red/orange
color.



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